Table 1.
The fish types, their simplified name used in the paper and the accession number of their mitogenomes as submitted to Genebank.
Table 2.
Primers designed for amplifying mitogenomes.
Fig 1.
The phylogenetic trees based on the nucleotide sequences of 13 protein-coding genes from 20 mitogenomes using the NJ method.
aF2-1 represents F2 hybrids of (MA×MT)× (MA×PP), (MA×PP)×MA, (MA×PP)×(MA×MT), (MA×PP)×(MA×PP) and (MA×MT)×(MA×MT); bF2-2 represents F2 hybrids of (MA×MT)×MA. Numbers above the nodes represent bootstrap values.
Table 3.
Base composition of 17 mitogenome sequences.
Fig 2.
Gene map of Megalobrama amblycephala (♀) × Megalobrama pellegrini (♂) mitogenome.
All the bream species and the hybrid individuals shared the same gene arrangement and possessed similar gene sizes. Those genes encoded on H/L-strand are shown outside/inside the circular gene map, respectively. The Inner ring indicates the GC content. The figure was initially generated with OGDRAW (http://ogdraw.mpimp-golm.mpg.de/) and modified manually.
Table 4.
Comparative analyses of sequence similarity and variable sites.
Fig 3.
Percentage of nucleotide variation in different genes/regions of mitogenomes in F1 and F2.
There were no variation site found in the cox2 and atpase8 genes of F1 hybrids, and the Dloop region, tRNA, 12S rRNA, nd2, cox2, atpase8, atpase6, nd3, nd4l, nd5 and cytb genes of F2 hybrids. The variation rates here refer to the average variation rates.
Fig 4.
Synonymous and non-synonymous mutations of different hybrids in F1 and F2.
There were no synonymous mutations found in (MA×PP)×MA, (MA×PP)×(MA×PP) and (MA×PP)×(MA×MT).
Table 5.
The dN/dS values between the F1 hybrids and their own female parents.
Table 6.
Characteristics of M. amblycephala (♀) × M. pellegrini (♂) mitogenome.
Table 7.
The conserved consensus sequence in D-loop region of the 17 bream fishes based on the structure of the D-loop region in other fishes.