Figure 1.
Phase contrast image of a seta in a PBMC culture from buffy coat (a non-exposed donor) after 24 hours of incubation.
Mononuclear cells adhere along the setae.
Figure 2.
Setae from Thaumetopoea pinivora larvae and their interaction with PBMC.
A. A larva resting on the ground. Note the dark, round areas (called mirrors) on the back containing the setae; B and C. Cytospin preparations of lymphoid cells cultured with setae for 24 (B) and 72 (C) hours, respectively, stained with May-Grunwald Giemsa showing adhesion of PBMC to setae and degradation of setae.
Figure 3.
Cytospin preparations of PBMC after 6 days of incubation.
A and B represents a donor earlier exposed to setae, C and D illustrates not earlier exposed donors. The microscopic view indicated no difference between exposed and non-exposed donors. In A and C mononuclear are seen cells attached to the setae B and D the shows patterns of disintegrated seta. The cultured PBMC and setae were collected by cytospin and stained with May-Grunwald Giemsa.
Table 1.
Optimum of PBMC cell proliferation was at 400 setae/micro-well.
Table 2.
Setae and setae extracts did not stimulate enriched T-lymphocytes under conditions where anti-CD3 was a strong stimulator as measured by 3H-thymidine incorporation.
Table 3.
Setae and setae extracts stimulate the expression of CD3 and CD25.
Figure 4.
Setae and setae extracts stimulated proliferation of PBMC from setae exposed persons.
Here a multilevel model estimates for mean DNA synthesis by exposure in four non-exposed (hollow dots) and six exposed (solid dots) individuals. The vertical bars indicate the 95% confidence intervals.
Table 4.
Chitin enhances the stimulation of DNA synthesis in PBMC.